You have answered your own question. Proper chromatography methods require detection methods which are applicable to the samples under analysis. You may want to review the types of instruments, detectors, columns and materials available at your school before deciding on a final approach. Have someone in the school's analytical instrument lab assist you.

For HPLC samples with weak or no chromaphore, you will need to use a detector that does not rely on wavelength based light absorbance such as an RID, ELSD, CAD or MS, to name a few. Put a little time into researching this basic question so you will better understand the uses and limitations of these methods and detectors. I often suggest my students start with a keyword search on the web (GOOGLE or BING) to find examples and articles to review. This is one of the best ways to learn. Once you have familiarized yourself with the basics, be sure to ask your teacher for help in setting up and running some example methods.

BTW: You may also be able to derivatize some of your samples and use GC/FID or GC/MSD as an alternative to HPLC (only if the samples are appropriate).

You have answered your own question. Proper chromatography methods require detection methods which are applicable to the samples under analysis. You may want to review the types of instruments, detectors, columns and materials available at your school before deciding on a final approach. Have someone in the school's analytical instrument lab assist you.

For HPLC samples with weak or no chromaphore, you will need to use a detector that does not rely on wavelength based light absorbance such as an RID, ELSD, CAD or MS, to name a few. Put a little time into researching this basic question so you will better understand the uses and limitations of these methods and detectors. I often suggest my students start with a keyword search on the web (GOOGLE or BING) to find examples and articles to review. This is one of the best ways to learn. Once you have familiarized yourself with the basics, be sure to ask your teacher for help in setting up and running some example methods.

BTW: You may also be able to derivatize some of your samples and use GC/FID or GC/MSD as an alternative to HPLC (only if the samples are appropriate).

For UV inactive compounds you can use ELSD detector.

Please look at the following below links which may help you in your analysis:

https://lipidlibrary.aocs.org/lipid-analysis/selected-topics-in-the-analysis-of-lipids/detectors-for-hplc-of-lipids-with-special-reference-to-evaporative-lght-scattering-detection

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049412/

Thanks