As the title, for what specific reaction, PBS buffer should be 50mM as half of 1x PBS, and for what specific reaction PBS buffer needs higher concentration?
PBS = phosphate-buffered saline. It varies in composition, but often the composition is not specified in the methods section of papers. The optimal concentrations of the ingredients depends on the situation.
The concentration refers to the phosphate ion. The amount of cation necessary depends on the desired pH. Standard PBS is 100 mM phosphate with a pH between 7 and 7.4 in a 8-8.5 g/L sodium chloride solution, which is similar to human blood. The NaCl has no effect on the pH, which is determined by the ionization state of the phosphate.
Salt composition, pH, and concentration effects the folding of proteins and activity of enzymes. Therefore, the buffer determines enzyme activity measurements and must be specified for standardization purposes. Typically, the specified buffer system is the optimum found for that enzyme, but sometimes is just what the investigator had on hand.
The PBS concentration it will depends on your protocol, if you don’t find any kind of report about the concentration, you should select the concentration that provide to you the better condition for the assay that you want perform
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