when there are 3 isolates suspected as Saccharomyces, how can one screen for invertase enzyme producers using culture based methods or using reagents.
Salami Abimbola when it comes to culture methods which are used to screen yeast invertase procedures please do find the links and am sure you will like them as well
Article Experimental design of medium optimization for invertase pro...
Article Stimulation of extracellular invertase production from spent...
https://www.omicsonline.org/open-access/production-of-invertase-by-aspergillus-niger-under-solid-statefermentation-using-orange-fruit-peel-as-substrate-2329-8863-1000247.php?aid=83079
https://www.rsisinternational.org/IJRSI/Issue27/35-40.pdf
Hello you can use 2-5% sucrose solution with addition of 1ml of your culture liquid. Incubate it at 30, 40, 50, 60, 70*C, and than analyse for detection of reducing sugars by Benedict's reagent or any available method like Fehling's test or Nelson-Somogyi method.
1. You need to prove the enzyme intra or extracellular.
2. For enzyme activity calculation you need to analyse also protein content in your culture solution by Kjeldahl method or Lowry method for calculation of your enzyme activity per gram of protein.
Look for thermostabile invertase.
- Badges
- Science topic
- Similar topics
- Filing