Main criteria to select a restriction emzyme.
if we use any specific micronutrient in excessive amount in broth medium to grow bio-control fungi, so is it possible that thus specific micronutrient affect the secondary metabolites production...
21 August 2023 4,053 3 View
I want to modify a plasmid using crispr.. Would like to know if plasmids carry the proto spacer adjacent motif?
10 July 2020 7,736 0 View
A recent paper stated that virus could infect T lymphocytes which are negative to ACE2. Can you give your thoughts on it ?
15 June 2020 8,387 13 View
It may show difference in efficiency but is it recommended or usable method for extraction of bituminous.
27 February 2020 4,610 1 View
i have a list of proteins from mitochondrial fraction (from LCMS data) for which i need to annotate their diseases associated. if anyone knows databases in which the entire list can be pasted in...
04 September 2018 7,430 1 View
Hi all, Photophysical studies of a molecule which is under development was done by using uv-vis spectoscopy. By inctreasing concentration gradually, the intensity of one of the signal was...
15 May 2017 2,692 4 View
Can any one please suggest me reducing agent names for reduction of chlorides? for example: Cucl2+reducing agent >>copper or copper oxides. please give me the chemical equations and mechanism.
05 April 2017 5,340 4 View
Can any one suggest me minimum to maximum inhibitory concentration for kill the bacteria?
05 April 2017 8,593 3 View
Can any one suggest me binding agent names for mixing of powders?
02 April 2017 4,093 3 View
Can any one suggests me zinc and copper acetates in non-aqueous solvents and reducing agents for preparing the nanoparticles without any oxides under inert atmosphere.I need non-aqueous solvents...
20 November 2016 10,026 4 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
Several times by placing a restriction on the upper part of the membrane with the aim of preventing the drug from being added from this side, we tried to keep the drug at the top of the leaf, but...
29 June 2024 9,688 1 View
Hi everyone, I am currently trouble shooting my restriction digestion, so far it hasnt given me clear bands when I ran gel. I've been adding all reagents minus template, to make a master mix...
24 June 2024 1,171 3 View
I'm doing a 3C analysis shown in the picture linked and attached below with the Bait fragments labeled and the target fragments that will be tested for interaction with the bait labeled with...
02 June 2024 8,038 1 View
"How will it affect the material? Is a higher KAM value beneficial for restricting cracks, or is a lower value more effective?"
30 May 2024 9,211 0 View
Hi I am trying to set up the reactions with my plasmid, insert and RE. Usually the standard final volume is 20 uL or 50 uL (it depends on the protocol provided by the manufacturer) with 1 ug of...
28 May 2024 1,105 3 View
I'm a doctoral student with early experiences with recombinant protein synthesis. Since I still have to start my experiment and I do not yet know the quantities of DNA and plasmid that I will...
19 May 2024 5,171 3 View
these SNPs do not have restriction enzymes is it possible to do RFLP
15 May 2024 7,974 1 View
I have designed and assembled a 10kb plasmid containing SbfI and NcoI restriction sites flanking a promoter region, which I am attempting to excise and replace with other promoters. When I do a...
09 May 2024 2,553 1 View
Can we restrict the vertical and horizontal displacement in two directions for the lower semicircular in bottom hole of CT specimen and apply incremental displacement in the upper semicircular in...
09 May 2024 5,273 4 View
