Currently, I'm working on my senior research project that involves IHC staining of islets of Langerhans in rats. Prior to implantation (see BVP 8 CTL image attached), most of the islets demonstrate insulin staining (red) and good nuclear staining via DAPI (blue). However, in the explants after about 2-3 months of implantation (see BVP13/14 Explant image attached), the insulin stain remains in what we assume to be islets, but the nuclear staining is non-existent in these "islets". Has anyone experienced a similar problem in nuclear staining in explants? Or in islets? The green stain is for CD31, an endothelial marker, if that information helps.
Thank you for any help!
tags: islets of Langerhans, pancreas, implantation, explants, DAPI, staining, nuclear staining, lack of DAPI, insulin, CD31
Hi Nazar,
I understand that you are performing the same immunofluorescent procedure before and after implantation. The islets look very healthy before implantation, but it seems that after this procedure they loss their good shape. This could explain why you lack nuclear staining (the intraislet cells might have their nuclear membrane broken) but you still observe the cytoplasmic content in form of insulin. I suggest you to evaluate the viability of your islets.
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