Harshita B. Saksena

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Questions related from Harshita B. Saksena

What could be the reason for no detection of YFP in 35S:: Gene:: YFP lines in confocal in control as well as treatment conditions?

Out of 10-15 seedlings only 1-2 seedlings showed YFP fluorescence. However, in RT there is 5 fold Overexpression in line 1 and 30 fold Overexpression in line 2. Even then, there is no uniform YFP...

23 September 2023 3,620 0 View

How to reduce non specificity of anti GFP ab290 ( abcam) in western blot?

I have constructed a line of a stress inducible gene having native promoter::CDS:: GFP in Arabidopsis. The molecular weight of protein of interest+ GFP is 51kda. However, while performing western...

26 May 2023 6,547 3 View