Dear Ashish, you may do an in-silico analysis using http://www.cbs.dtu.dk/services/SignalP/. Watch out whether you get a change from a signal peptide (which is cleaved cotranslationally) to a signal anchor (which is not cleaved and thus your protein remains integrated in the membrane). These are predictions only, they give you an idea but the ultimate answer can only be given experimentally.

Dear Ashish, you may do an in-silico analysis using http://www.cbs.dtu.dk/services/SignalP/. Watch out whether you get a change from a signal peptide (which is cleaved cotranslationally) to a signal anchor (which is not cleaved and thus your protein remains integrated in the membrane). These are predictions only, they give you an idea but the ultimate answer can only be given experimentally.

Depends on several factors ... If this three amino acids comprising the binding region or receptor activation ... if it is critical for defining the tertiary structure. The peptide may lose the initial activity and acquire new functions. Physiologically proteins and peptides are cleaved by endoproteases as a form of regulation, and that cleavage can be represented by loss of few amino acids, 2, 3, 4 ... Nothing in the literature on the peptide in question? Good luck!