ANS dye is binds to hydrophobic patches on protein molecules. When I interact ANS with BSA in Phosphate buffer saline, I obtain two emission peaks at 446nm and at 517nm. What could be the possible reason for getting these two peaks?
In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.
28 July 2024 734 0 View
Can anyone comment on the following concerns I want to synthesize Ag doped GO-Fe3O4 nanocomposite in situ using plant extract as green method for Ag+ reduction. What will be the effect of plant...
23 May 2024 5,244 2 View
I submitted article, received comments, submitted revision. Now waiting for editor decision. Shall I wait for editorial decision or should put request in advance for waive off of article...
14 April 2024 1,935 1 View
We want to synthesize compounds with high quantum yield, but before starting synthesis we want to predict it computationally.
30 November 2023 7,355 2 View
Untreated cells are simply cultured cells. Do they indicate cell damage in daily cultured wild-type cells too?
23 September 2023 4,858 1 View
Has anyone worked with this antibody?
23 September 2023 4,618 0 View
Can you explain to me why BSA works better than Milk as a blocking solution for GTPase proteins?
22 September 2023 2,155 2 View
I have tried various transition metal catalysts of Zn/Cu/Al/Fe/ as Lewis acids, but no product.
20 June 2023 2,055 1 View
I am analyzing UPLC-MS data, where I don't have standards and performing a non-target screening of small-molecule compounds. How can I convert the mass ion intensity which is in percentage to ppm...
18 June 2023 2,164 2 View
Which freely available databases/tools/web servers can analyse LC-MS data to identify compounds and metabolites based on their m/z ratio values?
31 May 2023 2,621 5 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
Do these peaks in the curve indicate primer dimer or non-specific products?
01 August 2024 7,466 0 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...
30 July 2024 435 3 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM or SEM images?
25 July 2024 5,482 2 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
What is the future scope of acoustic emission?
17 July 2024 1,510 1 View
I do not know much about FTIR! The material Benzethonium Chloride USP was compared to a reference standard in regards to FTIR, as per the USP testing: Identification B. Infrared absorption (FTIR),...
16 July 2024 6,918 7 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View