Size-exclusion chromatography for the isolation of EVs?

Dear all, I am currently doing research on extracellular vesicles (EVs). I'd like to improve the isolation method by trying out size-exclusion chromatography (SEC) compared to serial...

07 September 2020 4,412 5 View

You can support me in a study on COVID-19?

Hello, I am a psychological researcher. We are conducting an investigation on COVID-19 and I hope to have your support....

17 July 2020 8,402 13 View

Which is the optimal optical density for toxical recombinant protein expression in E.coli BL21 (AI)?

Hi everyone. I am trying to express and purify a recombinant antimicrobial peptide using as a host cell E. coli BL21 (AI) (employing L-arabinose and IPTG). Because this peptide can be toxic to...

19 February 2020 4,888 4 View

How reliable are third-party pollsters such as SurveyMonkey, Qualtrics and QuestionPro for your thesis?

I do understand they are being used in consumer or market research. But what about if you engage them for your thesis? Are they reliable? Because of time constraints and for convenience, some...

13 December 2019 493 3 View

Is it reasonable to perform Free Energy Landscape (FEL) anallysis on molecular dynamics simulations of 50ns?

Hello everyone, I'm having an issue with a review of a paper. I performed 50ns MD simulations on docking poses to know if the predicted poses were stable. Among the literature doing similar...

24 October 2019 4,608 5 View

Can LiCl precipitation be used in purifying low RNA concentration (30ug/ml) ?

RNA which has been extracted from blood collected in Heparin tubes are not showing any amplification after cDNA synthesis, in comparison to Blood of same patient collected in ETDA tubes. Any...

25 September 2019 9,026 2 View

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07 August 2019 2,592 2 View

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04 August 2019 5,642 2 View

I have received a ginger extract, and it is written concentration 25%, and it is one liter. so what does it mean?

My research is to study the ginger effect on breast cancer cells. so how to know the best concentration to treat the cells with it to study the effect. Note that i will treat it with different...

16 February 2019 4,419 3 View

Is there a survey I can use that asks people to rate behaviors?

I am currently looking for an existing survey that will help me ask students how they view behavior. I am thinking that I can ask them to rate how appropriate a situation is (e.g. crying in...

22 October 2018 9,145 1 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

Why are my western blot bands so distorted?

Hi guys I'm out of ideas here please help! I've never had consistent problem like this doing westerns before and I can't figure it out. The condition used: - First 4 lanes have 50ug protein in...

04 February 2021 2,297 3 View

Can we coat the pellet with graphite spray in place of silver paste for making electrical contact to measure the thermoelectric properties?

I want to coat the pellet because without electrical contact (may be due to the high resistance of the pellet), the V-I plot is not coming in the ZEM-3 instrument. I want to measure electrical...

24 January 2021 836 2 View

Control in microstructure with growth inhibitor?

Dear researchers, I wish to understand the role of grain growth inhibitor in controlling the microstructure of the ceramic pellet. How it controls the growth? Also, I wish to know the grain...

15 January 2021 3,859 3 View

How to make sure that ultrasonic lysis of E.coli is done completely?

What's the criteria of comolete lysis of E.coli when breaking them using ultrasonic? What phenomena can help to determine if the cell are broken completely? From smell, transparence of the lysis...

07 January 2021 1,911 3 View

How do I know if my E.coli cells are fully lysed from sonication ?

Hi everyone, I am doing protein expression via E.coli. I am required to lyse the e.coli cells via sonication to obtain cell lysate. I normally sonicate 1L cell pellet suspended in salt buffer at...

22 December 2020 9,675 7 View

Blow extrusion of PLA with the HDPE non rotary die. Is it possible?

Hello, I managed to run a small blown extrusion line, head diameter 70mm / screw 45mm. The machine is to be used for extrusion of sleeves from 200 to 500mm wide. Unfortunately, I have a draw...

13 December 2020 2,824 5 View

Does freezing treated cell pellet at -80C damages them?

Hello, I hope you are all doing well. I have a question regarding my treated cells group. I plan to do western blot and qRT-PCR for my treated cells. However, to save time, I would like to...

06 December 2020 1,390 5 View