What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

I am electroplating Ni on a stainless steel. At temperature and time should I anneal so that Ni thermally diffuses into stainless steel?

I am electroplating Ni on a stainless steel for abrasive application. At temperature and time can I anneal so that Ni thermally diffuses into stainless steel? My intention is to improve the...

20 October 2020 7,092 3 View

What are the hot topics in today's situation where an engineer should focus for betterment of society, e.g. Rural development, Solar farming, etc.?

We engineers sometime busy in doing research in countless unpractical, non urgent, not beneficial to society like areas for increasing count of our research articles. But we know an engineer is a...

17 July 2020 1,950 16 View

Relative telomere length calculation with Ct values?

10 May 2020 7,398 2 View

Can any one suggest me what are improvements i can do in clustering of wireless sensor network based on residual energies?

I have already implemented clustering algorithm for WSN(IoT) based on remaining energies of CH's. But i need to improve algorithm for my academic project. Please suggest me the possible...

01 April 2020 3,357 5 View

Could ecological risk indices applied for water samples?

Could Pollution indicators such as degree of contamination index (Cd) and potential ecological risk index (PERI) proposed by Hakanson (1980) also considered for water samples to represent surface...

24 March 2020 7,345 4 View

What should be the average size of amplicon if I am using LBb1.3 (left T-DNA border) and RP (gene specific Reverse primer) against genomic DNA ?

Hello everyone! I am thinking to amplify T-DNA insertion region using LBb1.3 (left T-DNA border) and RP (gene specific Reverse Primer) against the genomic DNA of one of the T-DNA insertion mutant...

11 March 2020 1,239 3 View

Error while compiling WRF - "compile [-j n] wrf compile wrf in run dir (NOTE: no real.exe, ndown.exe, or ideal.exe generated)" Need solution ?

or choose a test case (see README_test_cases for details) : compile [-j n] em_b_wave compile [-j n] em_convrad compile [-j n] em_esmf_exp compile [-j n] em_fire...

06 February 2020 810 8 View

Which software is mostly used in industry for static structural analysis?

Most used CAE software used in industry for static structural analysis.

25 January 2020 6,260 3 View

Urgent- Historical data of FTSE 100(UK) NIKKEI 225(Japan) NYSE composite (USA) BSE Sensex for time period 2000-2019?

Can anyone help me in providing historical data of FTSE 100(UK) NIKKEI 225(Japan) NYSE composite (USA) BSE Sensex for time period 2000-2019. In yahoo finance, data is downloading in unknown file...

27 November 2019 6,731 3 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View

What is the method of removing RNase contamination from a filter paper surface?

I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...

12 February 2021 3,926 3 View

How do I stop losing peak shape with Waters T3 Atlantis Column?

I am using this column with a gradient of acetonitrile vs a phosphate buffer. I clean the column after every run with 2%ACN:98%H20 and store in 30% ACN. I've noticed that my peaks are getting...

10 February 2021 9,181 1 View

How to prepare OH- terminated Si Wafer?

Hi, Folks. I am working on the ALD project to deposit Al2O3 on the Si wafer from TMA and Water. I have struggled with the preparation of OH- terminated Si wafer. I have both Si(1 0 0) and...

08 February 2021 8,792 2 View

How to clean up an already pure protein from grwoth medium?

Hi all, I have got a protein, expressed in P. pastoris vector, more than 97% (SDS-page) pure in the culture medium after cells separation. Despite the protein expressed has a his-tag, Nichel...

04 February 2021 5,173 6 View

Can evaporation of PCR reactions lead to contamination in the cycler?

The whole volumes of my PCR reactions (10 uL) evaporated during the cycling (honest mistake, I know why it happened and how to fix it - wrong PCR caps used). My question is, could this leave some...

23 January 2021 3,887 4 View

Can anyone suggest an RNA extraction protocol from grape berries?

I am trying to extract RNA, from grape berries of a red variety, for qPCR (to study gene expression). I have tried nucleozol and a CTAB protocol (with b-mercaptoethanol+boric acid + cleaning with...

18 January 2021 8,857 2 View

I need to do a qRT PCR but there are not available filter tips?

Due to the COVID situation it is nearly impossible to find filter tips. Is it possible to do it without filter tips by making sure to clean the pipette with RNAse zap before use and working...

17 January 2021 8,839 3 View

Why do I have good performances for the test set but bad performances in predicting new data ?

Hello :) I'am trying to predict churn for the year of 2020 based on historical data from 2014 to 2019 on R Studio. The aim is to predict churn probability for every person. At the beginning...

13 January 2021 5,565 7 View