Hello all,

First time poster although I have been using this site to read and compare protocols.

I am going to set up a slot-blot analysis to verify differential gene expression among 48 cDNA clones. I want to standardize the concentration and volume of DNA added so that each slot will receive roughly the same amount of DNA onto the membrane.

Is there a recommended amount that has worked best? I was thinking around 50 ng DNA suspended in 50 ul of TE (1 ng/ul). Is this too low of an amount?

Thank you in advance