What causes VRBGA to turn yellow after culturing a sample on it and incubating for 24 to 48 hours?

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Can you tell me some good materials science journals with impact factor less than 1?

Materials science journals

09 July 2020 4,774 5 View

What would be the fast and smart strategy for writing a 40,000 word PhD thesis (dissertation), within 1-2 months ?

Dear researchers, I would like to hear and learn from your suggestions based on your general experience, during the course of your doctoral thesis writing.

20 May 2019 1,637 14 View

What theories can be used to determine the impact of participation on subjective well-being? What independent variables of participation can be used?

I am writing a research proposal and proposes to run a simple linear regression on impact of participation (independent variable) in social policy on well-being (dependent variable) of older people.

05 June 2017 1,324 3 View

What is the difference between cerebellar ataxia and cerebral palsy?

Both involve the loss of muscle coordination and balance. But how do the symptoms of cerebellar ataxia differ from that of cerebral palsy?

18 July 2016 9,166 8 View

How is Genetic Search is implemented for attribute selection in Weka?

The Genetic Algorithm can be modified in different ways. Even Goldberg's book, Genetic Algorithms in Search, Optimization and Machine Learning, specifies various enhancements that could be done...

11 April 2016 5,289 2 View

What is the proportion of the muscles active during gait?

I know that a large proportion of the muscles in the body are active for a person during her/his gait. But I'm not sure exactly (or approximately) the value of the proportion in percentages with a...

09 March 2016 531 9 View

Can WEKA be used to make two predictions on a given set of data?

Generally, a machine learning system would only make a single prediction among available classes (classification) or predict a single real valued number (regression) based on the given set of...

28 January 2016 5,321 6 View

What is the difference between clinical gait analysis and general gait analysis?

Apart from their apparent difference -- the area of applications differ -- what may be the precise differences between clinical and general gait analysis? To which degree is one associated with...

27 October 2015 2,292 9 View

How can you prove the absence of mathematical relation between ciphertext and plaintext?

The inventors of the AES Rijndael state that there are no 'linear' mathematical relations between plaintext and the corresponding ciphertext when passed through Rijndael. How can you prove this...

30 May 2014 9,389 2 View

How do you find self-similarity between two handwritten signatures?

How can one find the self-similarity between two genuine handwritten signatures signed by the same person? Or, more specifically, how could one find the degree difference between two given images?

18 May 2014 9,462 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View