Hello,
I work with root lesion nematode resistance in barley. For that, I need to perform resistance test under the greenhouse conditions. I have a DH population that I grow in the greenhouse.
Seeds were germinated on wet filter paper at 26°C for 1 day in the dark. Each seedling was placed in a 20 cm3 tube [12 cm (H) × 2 cm (∅)] filled with steam‐sterilized sand. A 20‐μm sieve, fixed at the bottom of the tube, prevented root outgrowth and inhibited nematode loss. The tubes were filled with heat‐sterilized sand and wetted with nutrient‐rich water until they were fully soaked before sowing. The tubes were placed in special holders in the glasshouse on an irrigation system.
The plants were fertilized with a nutrient solution as described by Marshall and Ellis (1998) with the following modifications (g/l): 1.40 g Ca(NO3)2 × 4·H2O; 0.11 g NH4NO3; ; 0.29 g K2HPO4 × 3·H2O; 1.5 g K2SO4; 0.96 g MgSO4 × 7·H2O; 0.96 ml trace element solution. The pH value in the solution was adjusted to 6.4 with 25% sulphuric acid.
Light conditions in the greenhouse: long day conditions (16h light, 900 μmol m-2 s-1, Son-T Agro 400 W, Koninklijke Philips Electronics N.V., Eindhoven, Netherlands)
Problem: As shown in the picture, the leaves are turning yellow. The yellowing process starts from the tip of the leaf and then it spreads over the whole leaf, eventually killing the plant.
Any ideas how I can solve this problem?
Thanks in advance.
-Smit
As it appears from the description it is not an infection related problem and you also have used the trace element solution thus should not be a nutrient deficiency either. But the physiological yellowing might be due to the over irrigated condition. In colder weather the transpiration is reduced thus causes a localized physiological stress on the roots. As you described, to prevent the nematodes from the tubes, a sieve was used, this might have caused the water stress too. Another reason might be the slow uptake of nutrients by stressed roots or the colder temperature. The latter seems less likely since you are growing plants in green house. Try to use foliar application of nutrient solution/fertilizer and intermittent watering.
Yes, it can be water-logging or Potassium or Molybdenum deficiency
The yellow color is due to a lack of nutrients such as nitrogen, so you have to control the doses for all nutrients used in this experiment.
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