What camera do you like for in vitro ephys?

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How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View

How long takes to a batholith to crystallize?

How long would it take for a 'normal' granitic batholith to crystallize? Are there any calculations on it?

12 July 2024 7,436 0 View

How will you write a literature review on impact of galamsey on agricultural land in Ghana?

A research proposal on impact of galamsey on agricultural land in wassa

12 July 2024 6,478 3 View

Why my comet have halo shape in comet assay ?

Hello every one, I am trying to perform comet assay. But my comet look like halo (cf pictures) and I have no idea why. Anyone has the solution ? I use the protocol and reagents from biotechne :...

24 June 2024 4,184 0 View

How can I properly identify hot and cold pixels manually or automatically to calculate the SEBAL model from Landsat images using GRASS GIS, R?

How can I properly identify hot and cold pixels manually or automatically to calculate the SEBAL model from Landsat images using GRASS GIS, R, or MATLAB?

18 June 2024 3,582 1 View

What are the key factors influencing the adoption and implementation of Building Information Modeling (BIM) in sustainable construction projects?

What are the key factors influencing the adoption and implementation of Building Information Modeling (BIM) in sustainable construction projects, and how do these factors impact project...

14 June 2024 202 4 View

How do financial ratios influence decision-making processes in construction management?

Financial ratios are critical tools in assessing the financial health and performance of construction projects and companies. Understanding their influence on decision-making processes is...

14 June 2024 1,639 3 View

How can zoning policies and urban development regulations be optimized to promote vertical development and enhance land use efficiency?

Understanding how zoning policies and urban development regulations can be optimized to promote vertical development and enhance land use efficiency is crucial for addressing the challenges of...

14 June 2024 6,311 12 View

What is urban noise pollution abatement management in details?

I need a clear and concise explanation

06 June 2024 6,682 3 View

Exist design guidelines for full-scale aerobic granular sludge process?

Are there any (national) technical guidelines for the process design of a full-scale water resource recovery facility applying the aerobic granular sludge process? The guideline should emphasize...

30 May 2024 9,287 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View