10 October 2013 1 5K Report

I start the analysis of gene expression and I do not know how to plan the experiment. I know about the dilutions, color compensation but I do not know what is the calibrator and what material I should use for that?

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What calibrator should I use for relative quantification in a dual probe hybridization assay on LC480 Roche?

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Inhibitors of DNase?

After isolation of genomic DNA I would like to get rid of the DNAse. I want to preserve short DNA fragments. Do you know how to do it? Any commercial kit available?

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