Hi,
I am a student working on my master thesis in biochemistry. In the research I do, we plan to increase the yield from producing the enzyme hSMUG1 (wt). One of the analysis I plan to do is to measure the zetapotential using the Zetasizer Nano, but the first time I tried to use it I "burned the cuvette". The cuvette I am using is DTS1070, and the buffersolutions I tried with is 50mM Tris pH 7.5, 300mM NaCl and 2mM beta-mercaptoethanol.
So my question is, can someone suggest a more suitable buffer solution for me to use during my analysis?
Sorry if something was unclear, I will try to elaborate if there are questions.
Best Regards Trond.
@ Trend, I think you may try for 10mM phosphate buffer or may go through the attached file.
Trond Bærheim
You actually 'burned' the (organic) sample on the electrodes rather than the (disposable) cuvette. You probably had an error about the conductivity of the continuous phase. Use the barrier diffusion technique and more dilute conditions. See attached and:
https://www.malvernpanalytical.com/en/learn/events-and-training/webinars/W110614ProteinZetaPotentialDiffusionBarrierMethod.html
https://www.malvernpanalytical.com/en/learn/knowledge-center/application-notes/AN110708NovelDiffusionBarrierTechnique.html
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