What are these weird circular shades under high content screening?

11 November 2018 0 2K Report

Hello everyone

I run high content screening with Perkin Elmer Opera Phenix andI found circular shades under bright field and digital phase contrast channel. Does anyone know what are those shades?

Badges
Science topic

More Jyun-Yuan Wang's questions See All

Factor score of dependent variable in JASP?

Hi all, I would really appreciate if someone can guide me how to obtain the factor score of dependent variable in JASP SEM analysis results.

20 July 2023 8,779 1 View

About electrochemical reduction of CO2 on copper foil?

Hi Everyone! I am currently trying to convert CO2 into C2 compound using copper as the cathode in a h-cell as everyone does. The electrolyte I am currently using is CO2 saturated 0.1 M ~ 0.5 M...

01 May 2021 5,852 3 View

Do you have any suggestions for me to modify my LS-DYNA model?

Hi, I use LS-DYNA to simulate the steel composite shear wall now. I use MAT_084/085 to simulate concrete, MAT_159 to simulate steel plates, and MAT_024 to simulate studs. The concrete section...

24 January 2021 4,070 1 View

Question about the ohmic drop compensation?

Hi everyone! I am working on measuring cyclic voltammetry (CV) and controlled potential electrolysis (CPE) for my cell. I understand the iR drop can be measured by several technics, such as EIS....

04 October 2020 1,331 8 View

Does copper metal react with sodium hydroxide?

Dear All Recently, I use deposit copper thin film on titanium and glasses via thermal vapor deposition under vacuum. I intend to use it as an electrode to do electrochemistry in alkaline...

03 July 2020 4,086 10 View

Question about the tafel plot for formic acid oxidation?

Dear All I have been working on electrooxidation of formic acid. I have two catalysts. One of them exhibits much higher activity (Catalyst A) than the other one (Catalyst B). Right now,...

17 January 2020 7,019 2 View

What will happen when high-pressure hydrogen injected into low-pressure air chamber?

High-pressure (5bar, maybe) hydrogen injected into low-pressure (1 torr, maybe) air chamber? Will it burn like a small convex flame front near the inlet, or hydrogen just enter the chamber, mix...

27 May 2018 4,710 1 View

Which reagent can be used for quenching Fenton processes before determining the TOC?

Sodium thiosulfate is usally used to quench with this. However, it has an effect on the TOC of its sedimentation.

29 January 2018 7,403 10 View

How do I avoid cell loss for analysis with flow cytometry?

Hi. everyone! Does anyone here familiar with indirect staining of intracellular proteins. Trying to stain106 cells, however, I only got ~500 cells. 90% cells loss :( Do any tricks to avoid the...

04 January 2016 7,809 4 View

Any advice with Finite-rate/Eddy-dissipation model with surface reaction?

Hi~ I got another problem with finite rate/eddy dissipation model using surface reaction. The surface reaction was activated by 1.Model→Species transport→check "Wall surface"2.Assign material in...

17 August 2015 596 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

People weight in Oaxaca Blinder Decomposition on R?

Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...

04 August 2024 6,033 0 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

Why running a restart analysis in Abaqus in Ubuntu OS gives an error as attached when running the same job in Windows doesn't give any error and runs?

I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...

29 July 2024 9,572 3 View