Is it possible to have repeated eigenvalues and linearly independent eigenvectors corresponding to the repeated values?

07 August 2016 7,791 12 View

What is the difference between the autocorrelation of a stationary signal and non-stationary signal?

If one takes the autocorrelation of a stationary signal, and then that of a non-stationary signal, then would it be possible to say which one is stationary from the ACFs?

31 December 2014 9,609 5 View

What is the geometrical interpretation of multiplying a matrix by the inverse of another matrix?

What happens geometrically when one multiplies a matrix A by the inverse of a matrix B?

31 December 2014 5,200 5 View

What is the advantage of multivariate change point detection over univariate change point detection?

In a multivariate time series, what extra information does performing multivariate change point detection provide than performing univariate change point detection of individual time series? Both...

11 December 2014 5,439 3 View

How should I handle zero R2 values?

Consider a time series (stationary or non-stationary). I fit a linear model of order 0, i.e., an intercept to the data. In the stationary case, this corresponds to the mean. Now, when I calculate...

10 November 2014 7,015 13 View

How can one identify a wrong parameter in a state space model?

I have a state space model - linear or non-linear. I know that there is (are) wrong parameter(s) in the model (the model structure is correct though). Is there a method to isolate the wrong...

09 October 2014 2,513 6 View

What is the discrete equivalent of complex conjugate poles on imaginary axis for continuous case?

If there are complex conjugate poles on the imaginary axis (+jw and -jw) for a continuous system, what would be the location of the poles of the equivalent discrete time system?

08 September 2014 4,124 5 View

What measures exist for characterising and classifying segments of data acquired over a period of time?

I have measurements from a system over a long period of time. During this time, the system has been in different states (for example, different controller tuning configurations). Now, looking at...

07 August 2014 3,337 7 View

Performance of EKF changes with noise in MATLAB?

When I change the seed of random noise generation in MATLAB, keeping everything else fixed, the performance of the EKF and also UKF (MSE, ACF of the estimation errors, etc.) changes every time....

01 February 2014 1,604 16 View

What is the effect of DC on fft in MATLAB?

What would be the effect of a dc component on the fft of a signal with time varying frequency content?

01 February 2014 4,364 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View