What are the standard protocols to quantify the total phenols, flavonoids and alkaloids of plant extracts which were prepared in non-polar solvent ?

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Marshall Arebojie Azeke

You must first evaporate the non-polar solvent, say, by using a rotary evaporator and then reconstituting the residue in water. The condition for evaporation will depend on the volatility of the solvent.

Zorita Diaconeasa

Hope this is usefull for you!

Article HPLC/PDA–ESI/MS Identification of Phenolic Acids, Flavonol G...

Vinesh Kumar

You can use Soxhlet extraction using solvent of different polarity.

Article Antifungal activity evaluation of different extracts of Berg...