HI sir, hope there is no region specific sero-diagnostic tests required for the particular pathogens, until unless the pathogen causes an outbreak in any region of the world the panel of tests required for the diagnosis would remain the same in any region it may be if the pathogen is same and not found associated with any outbreaks, where more specific and rapid diagnostic test would required to keep the spread under control .. hope the answer is useful to your query.

thanks

The common causes of Tropical Acute Febrile Illness (AFI ) in India would be Dengue , Malaria , Typhoid , Leptospirosis & Scrub Typhus . A primary care doctor should have the facilities to diagnose these diseases & he should be provided with relevant diagnostic kits , which are simple & can be done in small towns & villages . Falciparum malaria is common in central India , while leptospirosis is reported from south west India . It is essential that every state provides data on common diseases in their respective areas , so that relevant kits are easily available . There is a gross underestimation of the causes of AFI in various states of the country due to non availability of diagnostic kits .

I agree with you that sero-diagnostic kits should be available in every region of the country for common AFI & more kits are made available during an outbreak.

i understand sir, thank u for the reply.. i thought you are talking only about leptospirosis related serological diagnostic tests.. since you didn't mentioned about the AFI in your question, i answered as above. In our region (Mizoram), where am working, we included serological test kids for malaria (IgG, IgM), Widal and Typhi dot, Scrub typhus (common), dengue (sometimes).. leptospirosis is never been reported from this state (as per the State Health Department records).. i thought the leptospirosis is very common in Andaman and NIcobar islands as i found may articles published form the ICMR Unit from there..

hope this was useful sir. thank you

with regards.

Karuppasamy Pandiyan

Thanks for your valuable comments . This question was for the project on sero-diagnostic tests for leptospirosis in the union territory of Dadra & Nager Haveli . I am glad that you have the diagnostic kits for malaria , Typhoid , scrub typhus & dengue in your region . I request you to include kits for leptospirosis also , because this disease can be diagnosed only by diagnostic tests . In addition , co - infection of leptospirosis can occur along with malaria , scrub typhus & dengue . Leptospirosis is under reported in N.E.Indian states , except in Assam .

Good afternoon in relation to your question I only mention the diagnosis of leptospirosis, in Mexico it is done by means of MAT, in two serological samples with no less than 15 days of interval or titles higher than 1: 1280 in the first sample (it will be positive) which from my particular point of view is not indicated since the cutoff point is too high. In relation to this in next publications we comment on this particular point. The PCR test is also performed for Leptospirosis. These are the Official diagnoses. It is probable that others not recognized in the Official Regulations are used.

I hope that this short information will be useful greetings

Saludos

I agree with you that a titre of 1:1280 is definitely high for a first sample . A titre of 1:320 is adequate . The problem is that to obtain high titre , the sample should be taken after 10 days . In the period between 5 - 10 days , a titre of 1:80 or 1:160 is acceptable . This is done in our center . It is preferable to do 2 tests in this period . Elisa IgM is popular & sensitive rapid test & can be utilized with low titre MAT. I am sending my article , which may be useful to you .