A question about MALDI/TOF MS?

I am a Ph.D. student of microbiology in Iran. Actually, I have found a bacterial peptide that I want to identify that using MALDI TOF MS. I am looking for anyone with experience with MALDI TOF MS...

03 April 2018 9,078 6 View

Does anybody know what is the best method to isolate the bacteria from coral?

Does anybody know how to isolate the bacteria from coral? What is the best method?

09 October 2013 7,619 1 View

Does anybody know what bacterial strains can produce deacetylase more than other strains?

Does anybody know what bacterial strains can produce deacetylase more than other strains?

05 June 2013 4,375 0 View

Anybody knows about Pyrosequencing technique? and also its price for each sample?

I want to assess gut microbiota using pyrosequencing method. But I am not sure it is a best technique for my issue!

02 March 2013 6,550 3 View

Does anybody know what is the best DNA extraction kit for stool samples to get a good result with a low cost?

Does anybody know what is the best DNA extraction kit for stool samples to get a good result with a low cost?

02 March 2013 4,057 4 View

Hello everybody. I wonder if someone has the list of ISI journals and their impact factors to send me. ( preferably 2011) [email protected]

I have list of them but I need impact factor of 2010 0r 2011 years.

09 October 2011 5,359 0 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

Can anybody provide me sequences of SPF10 primers?

03 February 2021 1,053 2 View