Does anybody know what bacterial strains can produce deacetylase more than other strains?

06 June 2013 0 4K Report

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More Akram Najafi's questions See All

A question about MALDI/TOF MS?

I am a Ph.D. student of microbiology in Iran. Actually, I have found a bacterial peptide that I want to identify that using MALDI TOF MS. I am looking for anyone with experience with MALDI TOF MS...

03 April 2018 9,078 6 View

Does anybody know what is the best method to isolate the bacteria from coral?

Does anybody know how to isolate the bacteria from coral? What is the best method?

09 October 2013 7,619 1 View

What are the special diagnostic tests for uropathogenic E.coli?

I'm interested in the special diagnostic tests for uropathogenic E.coli.

09 October 2013 9,818 5 View

Anybody knows about Pyrosequencing technique? and also its price for each sample?

I want to assess gut microbiota using pyrosequencing method. But I am not sure it is a best technique for my issue!

02 March 2013 6,550 3 View

Does anybody know what is the best DNA extraction kit for stool samples to get a good result with a low cost?

02 March 2013 4,057 4 View

Hello everybody. I wonder if someone has the list of ISI journals and their impact factors to send me. ( preferably 2011) [email protected]

I have list of them but I need impact factor of 2010 0r 2011 years.

09 October 2011 5,359 0 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

Does anyone have a copy of the DIBaS dataset?

I am a student from Brunel University London, and I was looking to use the DIBaS dataset for my dissertation. However, recently it has not been accessible via this link:...

26 January 2021 5,185 4 View