What are the safety precautions that has to be taken while working on Legionella? Also can anyone please provide the necessary laboratory facilities?

More Suri Sowmya's questions See All

What happens to qPCR if extension step is not included?

I am trying to measure telomere length in human DNA samples through qPCR. In the protocol, they have not included extension step. Can anybody explain how a qPCR can work without an extension step?...

31 May 2024 514 2 View

What should be action by leadership when they find that HR personnel are acting based on personal opinions and personal issues with employees?

In many organisations , especially within the traditional style of operations,. HR sometimes plays the role of a destroyer, then a team builder. Personal grudges, personal expectations, , personal...

01 May 2024 9,256 5 View

Will centrifugation at low rpm (5000 rpm) will reduce DNA yield (especially DNA fragments)?

I am trying to perform Tunel assay for senescence. DNA fragmentation is one of the characteristics in senescence. I had extracted DNA from scenescent cells and performed Tunel assay. But the RFU...

22 April 2024 4,057 2 View

I want to use the graph of my matlab code in simulink as input to my logic...can anyone help me in doing it..??

i have generated a triangle wave in matlab and now i want to use that wave as input to my simulink model

19 June 2023 5,829 2 View

Has anyone used python to generate kinome tree?

I need to represent some data for kinome. Has anyone used python for kinome tre representation? Can someone lead me to an open-source resource for kinome tree generation?

07 June 2023 829 1 View

How can assess the medication adherence and compliance of syrup in pediatric patients??

Needs standard guidelines or scales or questionnaire's

31 May 2022 2,611 5 View

How to change harmonic force constants during simulated annealing in Gromacs?

I am trying to reproduce a paper which has followed a simulated annealing procedure with a simultaneous decrease in harmonic force constant. a) Starts at 60 K, force constant = 5.0 x 104 kJ/(mol...

21 September 2021 4,685 5 View

How much do we know about applying Benford's Law to detect cancer?

I am really keen to understand the applications of the physical law of unity in relation to the genetic aberrations related to cancer.

12 June 2021 872 1 View

Hello, I want to perform a questionnaire/survey to support my Analytical Hierarchy Process (AHP) results. What approach is best for this ?

I want to get second opinion from experts for my priority vectors. Should i use a AHP-format survey (asking for pair-wise comparisons) or a simple questionnaire asking them to rank every criteria...

15 May 2021 4,322 2 View

What could be good dissertation topics related to the European Energy diplomacy initiative?

I want to focus upon the external dimension of European Green Deal. Since I am a student of International Economics, I would prefer working within the field of International Relations/...

11 March 2021 8,981 4 View

Subculturing of colletotrichum capsici is not able to do please help me?

Hello all, i am working on Chilly anthracnose disease and the pathogen involved is colletotrichum capsici but the problem is occuring while subculturing, i can isolate pathogen from chilly fruit...

17 July 2024 1,610 2 View

What are the key risks associated with foodborne pathogens in imported leafy greens, the impact on public health and effect on regulatory framework?

I am currently writing a systematic review

15 July 2024 9,176 1 View

Many scientists have proven that the use of antibiotics against pathogens in dairy cows has a low success rate. What is the solution ?

I think that in such situations, hygiene measures are very important and the right milking machine is required. Which is the right one to obtain clean and safe milk with a high quality standard?

10 July 2024 4,362 0 View

I'm looking for a tool to evaluate the risk of bias in... Are there any recommended applications or platforms?

Dear researchers, In a systematic review (not a meta-analysis) compiling studies on various diagnostic systems against a specific pathogen. The most relevant parameters evaluated are the type of...

03 July 2024 9,069 3 View

What are the best criteria for detecting Healthcare associated infections, especially in case of urinary tract infections?

This is an important step for isolation of hospital pathogens.

18 June 2024 5,633 1 View

Is still the cryptococcus sp. considered as the top most critical priority pathogen?

In the year 2022 the WHO released a fungal priority pathogen list in which Cryptococcus neoformans being placed at the top most of critical priority pathogen. Is it still a threat to human...

10 June 2024 4,055 3 View

How do we Select SNPs from insilico analysis for experimental validation?

We have done in-silico analysis on SNPs (pathogenicity analysis, stability analysis, etc), which has given us the deleterious SNPs that were affecting protein structure. Now that we want 2 to 4...

28 May 2024 308 3 View

My mic (minimum inhibitory concentration) results are all negative, should i still compute the inhibition rate of the concentration?

this is about my thesis, testing the plant extracts against fungal pathogens but unfortunately all the results are negative

25 May 2024 401 4 View

Can I detect endogenous RNA in human plasma samples?

I would like to implement a endogenous RNA-based internal amplification control for a RT-qPCR assay used to detect viral pathogens in human plasma samples. Are there any detectable endogenous RNA...

22 May 2024 1,919 1 View

How to validate Insilco predicted SNPs with wet lab experiments ?

Collaboration and joint research Call: Recently we conducted studies on BRCA1, BRCA2, EGFR, and ESR1 mutations in cancer! Our computational approach identified potential pathogenic variations...

19 May 2024 2,012 3 View