Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

Sample modeling in ABAQUS software and its relationship with the real world?

About crack modeling in ABAQUS: Mode I (pure-tensile cracking) and Mode II (pure-shear cracking), these assumptions may be accruing in theoretical approach (ABAQUS) not practical in Lab....

19 February 2021 6,156 1 View

Making my research papers available for readers?

17 February 2021 8,533 2 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View

Does anyone recommend me the most reliable method to estimate temperature gradient?

Temperature gradient in well is sometimes estimated by either a temperature survey or running wireline formation tools. Meanwhile, well is generally full of a fluid whereby fluid movement is...

15 August 2020 6,653 4 View

How to assess bioclimatic stress among climate-displaced people as part of a study on social representations of diseases related to climate change?

Bioclimatic stress is one of the effects of climate change on human health. I would like to assess the probability for displaced persons identified in a slum population to have experienced, at...

17 July 2020 1,391 9 View

Two wells were drilled into the same formation. One was completed at a depth of 5800 ft........ ( Question in Description ) ?

Two wells were drilled into the same formation. One was completed at a depth of 5800 ft. When it was shut-in, it was filled with brine with a surface pressure of 70 psia. The second well was...

17 July 2020 1,230 4 View

Is Total Physical Response method useful in teaching language to deaf students?

Total Physical Response (TPR) is a method of teaching language or vo cabulary concepts by using physical movement to react to verbal input. The process mimics the way that infants learn their...

04 July 2020 9,344 2 View

Why is my Cronback's alpha low and what can I do to rectify it?

Hi all, I've used the ATHI scale to investigate attitudes towards homeless across two disciplines: Social Work and Computer Science. It has been validated. However, the scale only uses 11 items...

13 April 2020 8,344 8 View

O Comment on the sample size, sampling method, measures/data collection instrument and method used on ending homelessness lgbtq youth?

it is my team project and I am so confused. the team said we are going to use quantitative method for this part of our research project

29 January 2020 1,800 3 View

How to conduct a Chi-square analysis dealing with two independent groups?

Hello fellow scholars, I would like assistance with a Chi-square analysis dealing with two independent groups. In one research article I was reading, the researcher was studying US attitudes...

25 January 2020 7,039 6 View

O Comment on the sample size, sampling method, measures/data collection instrument and method used on ending homelessness lgbtq youth?

it is my team project and I am so confused. the team said we are going to use quantitative method for this part of our research project

31 December 2019 4,677 2 View

I have some XRD results in excel format with intensity and tetha values, how can I plot a reflectance curves with these results pls.?

I sent my soil samples for XRD and FTIR analysis and the results were sent in value forms. I need to analyse these values but handicapped as I do not have the required software for it. Can anyone...

31 December 2019 2,651 2 View

Where could I get some data about what normal people think about products for disabled people on streets??

Hi everyone! I am designing some products for disabled people. I would like to know when people see the products, like wheelchairs, harnesses, etc, how they respond and think about them. And I...

10 November 2019 8,245 6 View