Can I have done DRS test on thin film Glass substrate by carry 5000 UV-Vis NIR spectroscopy?

Please give the proper answer and how it is performed. Provide a short demo video if as soon as possible.

15 September 2020 821 2 View

What do you think about the superiority of a paper published in a high impact factor SCI journal & a low impact factor SCI IEEE Transactions journal?

30 November 2018 4,365 2 View

How Matrix is useful in projecting a three-dimensional image onto a two-dimensional plane in various computer programs?

Matrix plays an important role in computer science. It is useful in various computer programs, in projecting a three-dimensional image onto a two-dimensional plane.

16 May 2018 2,537 2 View

How does MOS technology is compatible to MTJ(Spintronic Device) while constructing STT-MRAM memories ?

04 September 2016 1,793 3 View

Can blotting effect if some amount of SDS-Buffer present in transfer buffer solution?

Can blotting effect if some amount of SDS-Buffer present in transfer buffer solution?

03 July 2016 725 4 View

What is main difference between Ct and Cq value? When do we use Ct value and when Cq In Real Time PCR?

03 December 2015 2,439 13 View

Which parameters to apply in my Master Curve?

I'm looking to develop a master curve considering three parameters, any suggestions for statistical model, like Sigmoidal function, any other such model which will be helpful?

25 January 2014 2,377 7 View

Why does andhra pradesh not have 66 kv substations ?

I have not seen any 66 kv substations in andhra pradesh

11 September 2013 411 1 View

Does temperature play major role in solar cell in power generation?

Seeking to find out whether temperature plays a major role in solar cell in power generation?

11 September 2013 5,609 9 View

How can I construct a vector to express a human protein for commercial use?

I want to express a human protein in bacterial expression system and eventually I want to commercialize this protein. I am not interested in commercializing the vector itself. Can anybody suggest...

19 May 2013 664 7 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View