Dear
Please look at these documents:
-Methods for Analyzing Diversity of Microbial Communities in Natural Environments
Fakruddin et al.Ceylon Journal of Science (Bio. Sci.) 42(1): 19-33, 2013.
https://cjsbs.sljol.info/article/download
https://www.rapidmicrobiology.com
http://ec.europa.eu/environment/life/project/Projects/index.cfm?fuseaction=home.showFile&rep=file&fil=BIOTAGENE
The identification of bacteria could be primarily done by morphological, biochemical and physiological methods. However, the molecular biological approach could hasten the identification process.
This is done by isolation of genomic DNA from the pure culture of bacteria followed by amplification of 16S rRNA gene fragments using 27F and 1492R primer sets. The amplified product is then purified and sequenced. The sequence, thus, obtained is matched with the reference sequences in NCBI database. The percentage match provides the information on how closely our bacterium is related to the matched known bacteria (Srivastva et al., 2015, 2017).
The service for sequencing or even the entire process i.e. from DNA isolation to sequencing is now provided commercially by some companies and institute like IMTECH, Chandigarh.
I hope you find the provided information useful.
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