Hey , how are you doing today,
I did splenic injection of tumor cells with C57BL6 to accomplish liver metastasis.
,but I don't understand some results of my experiment,,
The main thing is that..
All mice woke up 1~2 hours after surgery, and they all looked fine.
However, 5 mice (among 48 mice) got weaker, and died 24~48 hours later.
I have checked bleeding, but no sign of bleeding was found.
Infection?, If it is about infection, is anti-biotic gonna be helpful?
Please give me some possible reasons for these deaths
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[Brief procedures]
1. Anethesia by using Ketamine/xylazine mixure (9:1).
2. the spleen was exteriorized via 5mm abdominal incision, and cell line was injected.
3. after 1 min (to allow cells to flow into the liver), splenectomy was done, and homoeostasis was assured by ligation with suture and pressure with a swab
4. suture.
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Thank you for your time and concern, :)
Hwaijin,
Ketamine if given in excess may cause respiratory depression and xylazine may compound this effect and could be the reason for the death of mice. Even if given in right dosages ketamine and xylazine may sometimes exert these respiratory depression effects and mice may die as a result.
have u checked the inflammation caused by the surgery itself?
the amount of cells inserted?
what we did was to pour iodine after suture, wrap the mice with gauze and place it in the cage.
It was not becouse of the anestesia, since all mice woke up from it and the ones which died did it 24-48 later. It could be an infection (antibioticss will help, also careful treatment of the wound and matherial)
I used to do surgery to implant cells inside the pancreas of rats. I used different kind of anesthesia combination: Ketamine/Atropine. You have to recheck whether the dosage you were giving is right.
And I used to pour iodine before and after I stitched the wound. It helps to reduce chance of getting infection. And don't forget, before you make an open incision, you have to wipe some alcohol above the incision site.
Lastly, I'm not really sure if it helps or not, I used to cover the body of the rats with some thick paper towel to make sure that they are not cold. Because according to my personal experience, after an open surgery I felt really cold. So, I think it also happen to other mammals. (What a poor argumentation. haha) Moreover, it will help reducing chance of getting infection.
That's all I can tell. I hope it help. hehe
I had three rats that all of them had problem in one eye. After Ketamine an xylazin injection all of them died. what is the reason?
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