Isolating DNA from obligate fungi like powdery mildew from leaf surfaces can be tricky as the scrapping of fungal mat /spores from the leaf surface may contaminated with saprophytes?
Thank you very much Dragos Postolache and Ali Mahmoudpour for your insights.
Shaking heavily infected young tissues with fresh infection to isolate the fungus is a nice thought. But the quantity of dislodged fungal spores may be too less to process through conventional DNA isolation procedures. Further the shaking would also dislodge sparophytes from the leaf surface though in lesser quantities.
As indicated buoyant density centrifugation would be difficult, costly and time-consuming approach.
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Hi Srinivas
You can read many references to help you about methods extraction DNA from obligate fungi
https://www.ncbi.nlm.nih.gov/pubmed/26782502
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http://cdn.intechopen.com/pdfs/34844/intech-molecular_tools_for_detection_of_plant_pathogenic_fungi_and_fungicide_resistance.pdf
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https://www18.corecommerce.com/~opsdiagnosti445/files/Specific%20Isolation%20of%20RNA%20from%20the%20Grape%20powdery%20mildew%20pathogen%20Erysiphe%20nector,%20Epiphytic,%20Obligate%20Parasite.pdf
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https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1002230
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http://www.springer.com/cda/content/document/cda_downloaddocument/9789048197347-c2.pdf?SGWID=0-0-45-1120158-p174024884
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https://research.wineaustralia.com/wp-content/uploads/2012/09/CRV-99-23.pdf
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https://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2009.03896.x/full
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https://www.genetics-gsa.org/fungal/2017/Abstract%20Book%202017%208x10.pdf
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https://bmcgenomics.biomedcentral.com/track/pdf/10.1186/s12864-016-3369-8?site...com
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https://journal.frontiersin.org/article/10.3389/fmicb.2016.00403/pdf
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http://www.encyclopedia.com/plants-and-animals/microbes-algae-and-fungi/moneran-and-protistan/fungi
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https://www3.epa.gov/pesticides/chem_search/reg_actions/registration/decision_PC-119196_1-Sep-02.pdf
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https://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2004.01077.x/pdf
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https://onlinelibrary.wiley.com/doi/10.1111/j.1574-6941.2006.00102.x/pdf
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https://research.wineaustralia.com/wp-content/uploads/2012/09/CRV-99-23.pdf
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https://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2008.01864.x/pdf
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https://horticulture.ahdb.org.uk/sites/default/files/research_papers/CP%20099b_Report_Final_2013.pdf
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http://comp.uark.edu/~ashi/Ainong_UARK/publication/2011/Lilac_Alternaria_120-127%202011-0199.pdf
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https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100069
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https://www.biotechniques.com/multimedia/archive/00010/97221bm08_10209a.pdf
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https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0110897
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http://www.tsijournals.com/articles/molecular-characterization-of-alternaria-alternata-apple-pathotype-from-kashmir-valley.pdf
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https://content.ces.ncsu.edu/extension-gardener-handbook/5-diseases-and-disorders
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