I have run the TLC of crude acetone plant extract with solvent system of Chloroform, Hexane and Methanol in the ratio of 7:2.5:0.5 and can visualize 6 more spots under UV, 365nm.
We used the Thin-layer chromatography (TLC) with the Bauhinia variegeate L. extraction of active constitute (flavonoids and polyphenol). The detection in this method gave the spots were characterized by Rf- values and color under UV light.