Can anyone suggest the mRNAs that may play a role in osteoporosis or any other bone related disease?
Yeah I meant the mRNAs whose expression is down regulated.. Thanks Christopher..
I agree with Christopher, he has put it well as to how the genes may get modulated at the time of bone loss as it happens in osteoporosis.
I have a couple of datasets that I scraped myself with Python. One is a list of countries with a few figures on how easy they are to work with from a business stand point (days items are in...
28 January 2020 9,278 3 View
A literature search yields that the size of sericin protein isolated from cocoon of silk worms ranges from 20-400 kDa in SDS-PAGE. The number of bands can be attributed to the reducing effect of...
09 September 2014 293 3 View
We want to try the ribopuromycylation method for locating active translation in cells e.g. David et al., 2011. JBC. 286. 20688-20700
02 April 2013 4,296 2 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View
Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...
22 February 2021 9,969 3 View
I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...
22 February 2021 1,853 3 View
Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...
18 February 2021 1,518 1 View
There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...
18 February 2021 9,109 5 View
Hello, I I am validating a system to deliver mRNA using nanoparticles to a mouse model. I am initially using mRNA for firefly luciferase to be able to do live imaging in the mouse. After that, I...
17 February 2021 2,536 3 View
We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...
13 February 2021 9,174 1 View
Dear colleagues I have the following questions concerning the safety of mRNA vaccines targeting the spike protein of SARS-Cov2: 1) The vaccine elicits antibodies which block the peptide domain...
04 February 2021 721 6 View
Hello there, I'm currently trying to over-express a gene by transfecting the cells with a pcDNA6 plasmid containing my target gene. When I measure the mRNA for my gene of interest, the levels are...
03 February 2021 4,112 4 View