What are the mRNAs that decay during osteoporosis?

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Anyone who has worked with the FPGA board Artix Ultrascale+ XEM8320?

I need help in monitoring the ADC(Model LTC2268-14) data which is interfaced with Artix Ultrascale+ XEM8320 board.

27 February 2023 9,687 5 View

Why do I see two peaks on my MFI charts for APC stained Mucin-1 protein for a single celled population of CLL cells?

Background Info to experimental set-up: Mutated Mucin-1 is found to be aberrantly expressed in diverse cancers and has been detected in certain mature B-cell malignancies such as Multiple Myeloma...

23 December 2022 5,578 0 View

Are Mean Fluorescence Intensity (MFI) graphs used to represent expression of a protein in publications? Such as an overlay MFI graph from FlowJo?

23 December 2022 5,587 0 View

How do you analyze a data set with nominal and continuous variables?

I have a couple of datasets that I scraped myself with Python. One is a list of countries with a few figures on how easy they are to work with from a business stand point (days items are in...

28 January 2020 9,405 3 View

Are there any common fixes for ANSYS Rigid Dynamics partial solutions?

I am new to rigid dynamics and am recieving the following error message "Although the solution failed to solve completely at all time points, partial results at some points have been able to be...

12 January 2020 6,788 0 View

How can I extract permittivity and permeability from S-parameter in case of meta-materials?

Microwave filter Meta materials Parametric Extraction

26 November 2015 2,475 6 View

What is the basic principle for choosing solvents for GC-MS analysis?

Do the samples used for GC-MS analysis vary according to the compounds to be analysed? What if one is not sure of the compounds formed?

24 November 2015 3,195 12 View

Is detection of retinal vessels and detection of microaneurysms linked? Is there any reference or link that would be proper guide to classifiers?

Classifiers used for image processing tools?

09 November 2015 2,024 1 View

What is the molecular weight of sericin protein?

A literature search yields that the size of sericin protein isolated from cocoon of silk worms ranges from 20-400 kDa in SDS-PAGE. The number of bands can be attributed to the reducing effect of...

09 September 2014 396 3 View

Is the size of liposomes from DLS measurements always similar to that obtained from SEM measurements?

Hi all, In my experiment the liposome size is obtained from DLS and SEM measurements for the same formulation do not match. Why is that? Any reference links will be helpful. Thanks.

10 July 2014 8,815 12 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

How to transfect circular mRNA to T cells?

Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...

21 July 2024 4,322 1 View

What happens in snapTotal- seq?

What happens in snapTotal- seq? (Single-cell Total-RNA Profiling Unveils Regulatory Hubs of Transcription Factors https://www.nature.com/articles/s41467-024-50291-3) In this protocol, several RT...

18 July 2024 10,010 0 View

FACS markers to characterize rat bone marrow-derived macrophages?

Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...

15 July 2024 4,615 1 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View

Stable cell line generation: GOI is detected by qPCR but protein is not expressed?

Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...

11 July 2024 4,812 5 View

Abaqus 2023 - How to access element - scale for implementing a failure criteria with subroutines (UMAT, USDFLD, UEXTERNALDB, ..) ?

Hi all, Second question on the same or so topic. I am working on subroutines (UMAT and others) for Abaqus to implement Tsai-Wu failure criteria for non-composite structures (I work on long...

25 June 2024 2,955 1 View

What is the best protocol for bone marrow cell culture? Is it critical to isolate the mesenchyme cells in advance?

I need to set a protocol to culture the bone marrow cell. I appreciate it if anyone help me.

22 June 2024 7,562 2 View