How to extract peaklists from mass spectrometry raw file and do mascot search for my protein in ncbi?

I have a raw file of tandem mass spectrometry (LC-MS/MS) data. I want do online protein databank search for my protein identification and its homology.

30 January 2021 3,081 3 View

How can i save multi medical images after some preprocessing in a file ?

i have multi x ray images after preprocessing on it , i saved them as a vector in file .dat but this file not accepted in next stage (convolution neural network ). how can i save all image in...

27 December 2020 1,369 4 View

What is microbiology and its types,scope?

the study of microorganisms is called microbiology Microbiology is the study of microscopic organisms, such as bacteria, viruses, archaea, fungi and protozoa. This discipline includes fundamental...

13 December 2020 8,287 6 View

How can one calculate Gibbs energy and entropy in ab initio molecules dynamics?

Hello, I've been investigating a bunch of monomers which spontaneously react in water to form a polymer. I would like to know how to calculate the thermodynamics of such a system. On the surface,...

18 November 2020 5,595 3 View

How to make a solution of resazurin powder ???

I want to test chitosan nanoparticles as antimicrobial against pseudomonas How to use resazurin microtitre assay????

08 November 2020 9,533 2 View

Please suggest me EI compendex conferences with good acceptance rate?

I want to publish a paper at the EI-Compendex conference, please suggest a conference with a high acceptance rate. My field of research is IoT security and cryptography analysis.

19 October 2020 5,365 3 View

Why COS 7 Cells change its morphology to round shape (rounded nucleus and peri nucleus cytoplasm) after Transfection?

I have transfected COS 7cells to express Protein-GFP conjugate, transfection rate was very low (20-30 %) , but the cell morphology changed, many of the cells appear round, (nucleus round too)...

15 September 2020 5,324 3 View

How to separate mixture of two organic compounds using size exclusion chromatography?

My organic compounds have a molecular weight in the range of 2500g/mol and other is 1066.22 g/mol. aThe TLC results show three spots. But the main problem with my compounds are acid sensitivity of...

20 August 2020 6,119 3 View

Dear All. Can normal RNA extraction kit be used for RNA extraction from cell-free medium like culture supernatant, VTM, plasma, etc.?

I am asking specifically for RNA extraction for VTM containing COVID-19 SARS-CoV-2 virus particles. Will the extraction kit make difference in total or specific (viral) RNA yield? There are many...

16 August 2020 8,385 2 View

Should outlier analysis be done before PCA while trying to perform step-wise Regression? Also how to deal with outliers?

Currently I am working on a project wherein I need to predict the estimated new Selling price of IPL players based on their current base price and previous years' performance & sold price. I'm...

05 August 2020 4,254 6 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

How to construct different filler shape in MD simulations?

Is there any way to construct different initial filler shapes like triangle, square, rectangle, pillar, and tube in any simulation software.

20 February 2021 1,750 1 View

Blast analysis of RC,UHPC slabs using material model *MAT_72R3 in LS-DYNA?

20 February 2021 5,984 3 View

How can I measure the surface interaction or surface interfacial chemistry between polishing abrasives and upper surface of substrate ?

I am working on polishing abrasives. I need to do few characterizations ? which can provide the on time surface chemistry of substrate with polishing abrasive during optical polishing. I would...

18 February 2021 5,319 3 View

Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View