What are the microbes associated with common insect pests and how can we elucidate their immune responses with imaging flow cytometry?

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How long do poured agar plates (LB, mannitol salt, etc) last before they should not be used?

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Gram positive encapsulated rods from blood agar?

We are growing bacteria from environmental sources, found a gram positive rod that appears encapsulated when we do a gram stain. Rods are long, not short. We have some fungal contamination as...

05 June 2014 5,830 12 View

I am hoping to do flow cytometry on hemolymph from Periplaneta americana. Has anyone ever tried fixing these or other insect cells?

I've extracted cells via perfusion with saline (PBS) but would love any advice about whether to use a different form of saline (insect medium, carlson's saline?) and whether I should use an...

01 February 2014 4,927 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

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Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

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Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

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Advice on growing lactobacillus species in the lab?

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How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

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Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

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Subhadip Das

1st u have 2 check ta cytokine status

Jean-Philippe Buffet

Dear Robin,

I am working on microorgansms associate with bark beetles. A recent paper on Dendroctonus ponderosae described the bacterial community associated with this insect using metagenomic analysis, and may answer the first part of your question:

Adams et al., 2013. Mountain Pine Beetles Colonizing Historical and Naïve Host trees Are Associated with a Bacterial Community Highly Enriched in Genes Contributing to Terpene Metabolism. Appl. Environ. Microbiol. June 2013 vol. 79 no. 11 3468-3475