Robin Herlands Cresiski

4 Questions 5 Answers 0 Followers

Questions related from Robin Herlands Cresiski

How long do poured agar plates (LB, mannitol salt, etc) last before they should not be used?

They are not contaminated and have been kept at 4C. We have plates that were poured about 3 months ago (mannitol salt, LB, pseudomonas agar, etc) and want to know if they are usable and still...

06 June 2014 4,426 13 View

Gram positive encapsulated rods from blood agar?

We are growing bacteria from environmental sources, found a gram positive rod that appears encapsulated when we do a gram stain. Rods are long, not short. We have some fungal contamination as...

06 June 2014 5,848 12 View

I am hoping to do flow cytometry on hemolymph from Periplaneta americana. Has anyone ever tried fixing these or other insect cells?

I've extracted cells via perfusion with saline (PBS) but would love any advice about whether to use a different form of saline (insect medium, carlson's saline?) and whether I should use an...

02 February 2014 4,953 3 View

What are the microbes associated with common insect pests and how can we elucidate their immune responses with imaging flow cytometry?

I am working with undergraduates to do metagenomic analysis of microbes associated with cockroaches and also to examine the cockroach immune system in response to infection.

10 October 2013 4,878 2 View