What are the methods of sampling rhisospheric bacteria as well as the techniques of isolation and identification of these bacteria?

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Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View

Method for estimation of biomass using non-destructive method for ornamental plants?

Allometric equations are available for trees but couldn't find any method/equation for ornamental plants?

04 July 2024 2,646 1 View

How can CHARMM-GUI be used for protein-ligand interaction ?

My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.

24 June 2024 5,044 1 View

Viscosity or Complex viscosity should be used in the SOP for Malvern Zetasizer or carrying out DLS experiments for polymer samples?

Hi all, I am trying to conduct Dynamic Light scattering experiments on polymer sample using Malvern Zetasizer ZS. One question I have is to whether it is okay to use complex viscosity instead of...

11 May 2024 4,623 7 View

Could anyone provide me a spreadsheet to present the composition of sulphides in diagrams?

10 April 2024 6,290 0 View

How to calculate the Mean normal partial thromboplastin time ?

Is it like MNPT (geometrical mean of at least 20 healthy subjects) ? or it's the arythmetical mean ? can a pool of healthy subjects' plasma be considered as the reference APTT for calculating APTT...

01 April 2024 1,846 0 View

Crispr knockout validation?

I am doing crispr knockout in T cells. After transfection, I am trying to validate knockout by western blot and sequencing. with western blot, I can see truncated protein in knockout cells whereas...

30 March 2024 4,408 2 View

Crispr knockcout validation?

I am doing crispr knockout in T cells. After transfection, I am trying to validate knockout by western blot and sequencing. with western blot, I can see truncated protein in knockout cells whereas...

29 March 2024 5,969 1 View

What are teachers' motivation strategies?

This research aims to investigate the relationship between teachers' intrinsic motivation strategies and EFL students' outcomes in Algerian university

22 March 2024 3,670 0 View

Is energy minimisation necessary step before molecular docking for protein-ligand interaction?

I have to perform molecular docking on 2 ligands (A structure-absent in literature and B structure-sourced from PubChem) therefore, is energy minimisation necessary for both?

14 March 2024 7,560 3 View

Hello Researchers, Can anyone guide me on estimating the Leaf Area Index (LAI) of wheat using only weather and soil parameters? Is it possible?

I need to estimate the LAI of a wheat crop using only weather and soil parameters. Can anyone please help me?

28 July 2024 4,886 2 View

Markers to distinguish individual cardiomyocytes?

I recently isolated mice cardiomyocytes and found some are not individual cells. I tried to stain the cells with Wheat Germ Agulutinin, it did not work. Does anyone have recommendations on the...

20 June 2024 3,225 2 View

How top dressing of nitrogenous fertilizers can be done in mulched wheat and rice crops and how should FYM/compost be applied in zero till wheat?

02 June 2024 3,836 3 View

Gametocide for wheat?

We need to produce hybrid wheat seed for our experiment. Ca anyone tell me what's the best chemical agent for this purpose and where to buy the chemical agent? Thank you for your help.

27 May 2024 834 2 View

What are the essential data and calculations to be taken for the research that aims to increase soil Organic matter using mungbean as cover crop?

I am a researcher of Soil science department. 5 months ago I had planted wheat for my research. The research design was split plot design. it has 3 replications, each replication has 2 main plot...

05 May 2024 4,815 3 View

How can I extract DNA using Chelex100 from wheat common bunt?

Hi everyone, I'm trying to extract fungal DNA from wheat common bunt using chelex100 and I've tried several different protocols without any success. I've used different concentrations of chelex100...

29 April 2024 7,317 1 View

If the seeds didn't germinate well equally on every plot and i need data from 1m2 area only. can i sow seeds in 1m2 in gap filling for research?

I am a researcher of Soil science department. 5 months ago I had planted wheat for my research. The research design was split plot design. it has 3 replications, each replication has 2 main plot...

28 April 2024 7,432 5 View

What is the critical level of sulfur for wheat production in tropical soils?

I appreciate it if someone could get me references on the critical level of sulfur in tropical soils for wheat production.

15 April 2024 2,514 0 View

Which size of pots should be used for straw with soil and what should be ratio of straw and soil?

Corn straw with soil, how much quantity of both should be used and which size of pots should be? Crop is Wheat.

24 March 2024 6,296 0 View

Does anyone know of any published research work that addresses the topic of using rhizobacteria that promote plant growth through foliar inoculation?

I am interested in knowing if there is any precedent in the scientific literature on the use of plant growth-promoting bacteria obtained from the rhizosphere that have been inoculated into the...

24 March 2024 6,532 2 View

Bita Jamshidi

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0164533

Khaled Said Ali

https://www.mdpi.com/1422-0067/20/16/3989/htm

https://www.arccjournals.com/journal/indian-journal-of-agricultural-research/A-516

https://www.frontiersin.org/articles/10.3389/fmicb.2019.01639/full

Gonzalo Galileo Rivas Platero

Article A standardized method for the sampling of rhizosphere and rh...

Article Isolation and Analysis of Microbial Communities in Soil, Rhi...

https://bio-protocol.org/e1569

Rafik Menad

Bonjour,

J'espère que tu vas bien et mon message te retrouve en parfaite santé. Je te conseille de prendre contact avec : Toumi Mohamed , Zermane Nadjia

, Omrane Toumatia , ils sont très généreux et ils pourront t'apporter de l'aide.

A ton service en cas de besoin Meriem Richa

M. Latati

I think that you need to take in first a representative sample of the rhizosphere (as compared to bulk soil) ...