Dear Dr. Francien botha,

I know one article which will be very helpful to you. The author of this article Niko Radulović is already a member of Research Gate

Antimicrobial Plant Metabolites: Structural Diversity and Mechanism of Action.

Authors: Radulovic, N.S.; Blagojevic, P.D.; Stojanovic-Radic, Z.Z.; Stojanovic, N.M.

Source:Current Medicinal Chemistry, Volume 20, Number 7, March 2013, pp. 932-952(21)

Publisher: Bentham Science Publishers

https://www.researchgate.net/publication/233847733_Antimicrobial_Plant_Metabolites_Structural_Diversity_and_Mechanism_of_Action

Regards

SALMAN AHMED

Ph.D Fellow

Article Antimicrobial Plant Metabolites: Structural Diversity and Me...

Dear Dr. Francien botha,

I know one article which will be very helpful to you. The author of this article Niko Radulović is already a member of Research Gate

Antimicrobial Plant Metabolites: Structural Diversity and Mechanism of Action.

Authors: Radulovic, N.S.; Blagojevic, P.D.; Stojanovic-Radic, Z.Z.; Stojanovic, N.M.

Source:Current Medicinal Chemistry, Volume 20, Number 7, March 2013, pp. 932-952(21)

Publisher: Bentham Science Publishers

https://www.researchgate.net/publication/233847733_Antimicrobial_Plant_Metabolites_Structural_Diversity_and_Mechanism_of_Action

Regards

SALMAN AHMED

Ph.D Fellow

Article Antimicrobial Plant Metabolites: Structural Diversity and Me...

The second one article is

Antimicrobial Activity and Probable Mechanisms of Action of Medicinal Plants of Kenya.

Mwitari PG, Ayeka PA, Ondicho J, Matu EN, Bii CC.

PLoS ONE 8(6): e65619. (2013)

doi:10.1371/journal.pone.0065619

If my providing answers really helps you

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Tx Salman

I have the second article but the one of Niko will be very helpful.  You do not know of anything where researchers has used SEM or TEM to evaluate the effect on the cells?

Regards

francien

Thanks

I have done all the tests as descibed in these article, I want to know if anybody has investigate the mechanisms of action of natural compounds or extracts compared with that of antibiotics.  Does the extract demolish the cell wall or is the action on DNA synthesis or ribosomes.

regards

Francien

it depends on the particular bioactivity, but in general it involves penetration of active ingredients followed by stepwise interactions

 In general, Gram-positive bacteria is more sensitive than Gram-negative bacteria. The sensitivity of the Gram-positive bacteria compared to Gram-negative bacteria can be attributed to the cell membrane constituents, where the Gram-positive bacteria contains an outer peptidoglycan layer, which was an ineffective permeability barrier, whereas Gram-negative bacteria contains lipopolysaccharides in their outer

membrane, for this reason, the Gram-negative strains are more resistance than Gram-positive strains. (Reference in attachment).

Kindly, if you or anyone of the peer who is following this question have known a further explanation about the mechanical, please provide me with that because I'm working on this topic these days. Thanks

Dear Marwan

Dr Elisha and me, as his supervisor, have just completed a PhD study where we have done detailed SEM and TEM studies to study the effect of on a compund, extract and fractions on Gram-positive positive, Gram negative bacteria, Yeast and Fungi.  On each of the oraganisms the effect was different and w are busy with the final preparation of the publications.  We have presented some of the work at a microscopy conference and I am busy loading the abstracts on reseachegate. The different constitutions of the cell walls allow the different extraxts to penetrate into the cells or to lise the cells. We used controls to make sure it was not the effect of the solvent used to disolve the dried exract in that is causing the effect. i hope to submit these articles before the end of February. On some the effect was on the sellular content, others cell wall and even more effects.

regards

francien

In general, the cell walls of Gram-negative organisms, which are more complex than Gram-positive ones, act as a diffusion barrier and making them less susceptible to the antibacterial agents than the Gram-positive bacteria.

In Vitro Antimicrobial Activity of Acacia catechu and Its Phytochemical Analysis. AuthorS: Bhawna Sunil Negi • Bharti P. Dave

Kindly go through the below given RG links.

Article Antimicrobial Properties and Mechanism of Action of Some Pla...

Article Comprehensive review of antimicrobial activities of plant flavonoids

Article Antibacterial Activities and Antibacterial Mechanism of Poly...