Brake specific emission would be a good choice. I think there is no ideal condition for the evaluation but it is more about how you justify your choice of the engine condition. E.g. the one that represents real world operating condition of certain application?
These are the basis, but depending on case one can check other specifics, it depends on scope of test: from lubricant, from fuel and from environmental points of views
There are three main sections that need to be taken care of:
1. Fuel properties: Calorific value, density, and viscosity are very important. CV will be used in the calculation of many other parameters and density and viscosity will help with the flow properties. Other properties such as flash point, cloud point, cetane number, octane number should also be taken into consideration, depending on the type of fuel.
2. Performance parameters: brake specific fuel/energy consumption and brake thermal efficiency are very important. You can also evaluate cylinder pressure and temperature, heat release rate, etc.
3. Emissions: CO, CO2, and HC are important. NOx, PM, and smoke are also important for fuels used in CI engines.
The reason for the use of biodiesels is basically to reduce emissions while not underperforming. Hence it will be necessary to measure the emission parameters like CO, CO2, NOx etc., also performance indicators like BTE and BSFC will also be useful