Is there any reason why I can not order an oligo and use it directly as an insert for SLiCE cloning?

I was about to design the primers for SLiCE, when I realized the insert is so small I could order it as one oligo and it would not even be that expansive. As its quite some time ago, that I used...

18 March 2020 2,996 3 View

Ethically, what are your views on publicly revealing reviewer status after publication of the journal article?

I generally was taught that reviewers should keep their reviews confidential forever. I was invited to review an article that was recently published. It was then picked up by a major news source...

19 December 2019 9,463 5 View

Required Sample Size: Full Profile Conjoint Experiment?

Hi all, I am trying to compute a minimum required sample for conjoint experiment where every participant sees each attribute combination represented in a profile. I am planning for four to five...

03 December 2019 8,904 2 View

Looking for breastfeeding/infant policy statement or practice guidance from dietetic associations?

I have a policy statement from the British Dietetic Association, and both a policy statement and practice guidance from the Academy of Nutrition and Dietetics (USA). I can't find any other...

22 October 2019 892 10 View

Why are poetry and literary journals that publish it not in your data base?

I publish poetry in peer reviewed journals. These do not seem to be in your "data base."

05 January 2019 5,175 3 View

Are there any studies that explore the relationship between cuticle thickness and body size in species of decapod crustaceans?

This relationship is very obvious to me (think of lobsters), but hard to actually measure. I could not find any study which addresses this issue!

11 September 2017 1,995 14 View

Best way to clean CTFE equipment in order to avoid DNA contamination?

Dear colleagues, I received different pieces of lab equipment (mainly pestils and alike) out of CTFE and would like to reuse the same pestils without autoclaving them every time. I do not have any...

29 August 2017 4,356 2 View

What are the systems responsible for regulation and expression of behavior in birds?

06 January 2017 320 5 View

Optimization problem for PGLS in gls in R?

16 March 2016 3,942 2 View

How do I stop RIPA-insoluble, Urea-soluble protein fraction from precipitating in Western blot wells?

18 November 2015 2,156 7 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Any stable dye to color acrylamide hydrogel beads?

Hi! We are generating hydrogel beads(50-60um) using microfluidics as single-cell beads. To simply all the experiments (especially the centrifuge), I am looking for a dye. The dye should be stable...

14 January 2021 4,383 8 View