I have done Annexin V by flow cytometry to detect the effect of cytotoxic drug on MCF7 cell line and actually I found early and late apoptosis in the control group ( not treated with drug only RPMI media)
Does anyone have a reason for this ??
Marwa Kholief
yes dr. Tarek I have used PI & there was no necrotic cells, these group of cells weren't treated only media.
Did you mean that to use DMSO in one of the groups ??
Thanks a lot
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Emmanuel Ifeanyi Obeagu
Dear this could be linked to increased oxidation generating free radicals that overwhelmed the cells.
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