Would you please recommend a function in R software that could be used with FUNGuild output to merge different rows that have similar guilds?

Dear colleagues, I have an output of FUNGuild tool (ASVs abundance in different samples and assigned to guilds) and would like to merge the ASVs that had similar ecological guilds using R...

16 February 2021 4,292 7 View

Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

Why is the OD values not decreasing using 3 dilution in Indirect ELISA?

I am using indirect ELISA to measure the antibody response against Aeromonas salmonicida in Atlantic salmon. I am using 3 dilutions of serum 1:50, 1:200, and 1:400. The OD450 nm values measured at...

08 February 2021 9,049 3 View

What is the best etchant for severely deformed, high purity magnesium?

I have tried using acetic picral and 5% nital solution when observing the microstructure of severely deformed pure magnesium. Unfortunately, I keep getting similar microstructures (as shown below)...

20 January 2021 8,783 4 View

Can you recommend me chemical engineering journals with fees "scopes" ?

dear all would you help me to find a scops journal in the field of chemical engineering with fees as I want to publish in a short time. thank you [email protected]

23 December 2020 6,883 1 View

Sensitivity Coefficient in Uncertainty Calculations for HPLC assay method, UV assay methods and pH measurement?

Does anyone have a paper or any document for calculated sensitivity coefficients used in uncertainty calculations for HPLC assay methods, UV assay methods and pH measurement methods?? I know it...

21 December 2020 5,196 2 View

How to interpret PCA results in weka & how to extract features from it?

I'm working on KDD CUP'99 dateset. Training and test set are different datasets. after pre-processing I got 25 attributes. I applied 4 different classifiers through PCA in WEKA(classify tab). Now,...

20 December 2020 8,425 4 View

How can we define columns in 3D PLAXIS model for buildings existing near the excavation?

I started by a model using 3D PLAXIS to study the effect of deep excavations on existing buildings. The building was simulated as a surface plate with a surface load. Now I'm trying to do it as a...

29 November 2020 3,198 4 View

Add text at the beginning of a specific reference in Latex?

Hey researchers! The journal I'm submitting to wants datasets to be listed in the reference as [dataset] Authors; Year; Dataset title; Data repository ; Version; Persistent identifier (e.g....

18 November 2020 2,681 5 View

What criterion measures do you suggest to be used for a new children personality test?

I am working in a project that aims to develop a new non-verbal personality test for children. What king of measures/ intruments I can use to examine the convergent or divergent (or concurrent...

11 October 2020 1,960 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View