In a situation where serum sample collection, processing, storage and transport associated problems are ruled out, and controls in ELISA worked within manufacturer's limits upon strict adherence to instructional manual for the assay, what are the other factors likely to bring about low rate of anti-B19 IgG antibody?
IgG level will decrease overall during pregnancy, was the decrease in anti-B19 IgG normalized to total IgG levels or a reference IgG? There are multiple papers that have show this general decrease (One example: http://www.ncbi.nlm.nih.gov/pubmed/714321)
Thanks, James for this input. We did not estimate IgG levels but detection by ELISA using a set of controls (blank, negative, and positive), which of course, includes a reference IgG as positive control. We may have to do further studies, especially PCR on the serum samples to see if the low rate of anti-B19 IgG antibody among pregnant women in our study is due to depletion in immunoglobulin G level or not.
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