Please provide me with exact instruments and peripherals needed to establish an electrophysiology unit to measure the nerve conduction velocity in rat sciatic nerve of diabetic rats?
For a cheap/simple setup:
WPI or Warner Instruments may have what you need. If you want to keep the nerve suspended (requires longer nerves like from a rat) you can use a so called harvard chamber. If you mean a mouse nerve you better use suction electrodes.
With harvard chamber you only need to connect the stimulus and recordings leads with alligator clips. With suction electrodes which are fire polished glass capillary tubes you will need pipette holders with Ag/AgCl electrodes.
Stimulus can come from whatever source that could deliver a near square pulse in volts (10 V from a DA output of a acquisition board is fine).
For recording you would need a AD converter, controlled by a computer through a proper software (Axoscope, which is free, works).
Let me know if you need more details.
Best
Joao
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