I will be glad to receive a listing of all the scientifically appropriate experiments to conduct in order to indicate the anti-microbial activity of plant extracts. This includes experiment from extractions to sensitivity tests.
For Planktonic micro-organisms- First do agar well diffusion or disk diffusion to determine the antimicrobial effect of crude extract.
Then you can fractionate your crude extract and then determine the effect of those fractions separately. Likewise if you have facilities, you can go up to isolation of single active compound.
Apart from above steps, you can determine the MIC of your extract and/or fractions using a method like CLSI M 27A.
Dear Adolf, you can perform agar dilution method and broth dilution method to determine antimicrobial effects of plant extracts on pathogenic bacteria. As Gayan explained, you can perform your experiments according to CLSI.
Antimicrobial activities of plant extracts could be tested phenotypically using agar gel diffusion and MIC. Also, their effects on certain bacterial structure, product or virulence genes expression could be monitored by molecular ways.
agar dilution method, broth dilution method, and disk dilution method, According to CLSI document M27-A, and CLSI document M27-A3.
Dear Adolf, I think Gayan's answer takes care of your questions. However, if you need to further assess the bio activities of a plant extract, you may do a brine shrimp lethality test. You may also evaluate the synergistic/antagonistic effect of the extract when in combination with conventional antibiotics using different concentrations of the extract against your bacterial and fungal isolates.
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