Hi, 

The question is not very clear for me! you mean intensity calibration ? What do you mean by quantitative analysis calibration. Please give more details! 

Thanks 

Hi Mohammad Naji 

Thank you for your reply

I need to perform quantitative phase analysis using Raman Spectroscopy and PLS model. Yet, first I should prepare calibration set, i.e.,  a group of standards with known concentration/ratio and composition, and to capture their Raman spectrums.

I would like to know if there are any minimum requirements for these spectrums, e.g., spectrum resolution, number of accumulation, or laser exposure time.

I hope this clarifies my question.

Kind regards

Bashir

...If the standards are not sensitive the Raman laser (decomposition, oxidation, ..etc), I don't see any problem with acquiring Raman signal with good S/N ratio. First, is to try fast scans (1-2 s) with 2 accumulation and low laser power (< 3mW), then see what you get and change accordingly. If your standards are crystalline you don't have to acquire spectra for long time. The spectral resolution, depends on the wavelength, the optics and the grating you are using. You can quickly figure out if you already recorded a spectrum using the same setup conditions. If you set-up the conditions, you have to use the same for all the standards. You have to be very careful to probe exactly the same volume as you change the sample. Any change in volume, will affect your absolute intensities. 

One more important suggestion, is regarding the response function of your spectrometer. The intensity as function of the wavelength depends a lot on the grating and detector response. You have to correct for it before making any calibration with a set standards. 

Very useful information. Thank you very much.