These are the values that I have found in the literature.

Phase Estrogen pg/ml

Menses 42

Mid Follicular 68

Late follicular 159

LH/FSH surge 159

Ovulation 130

Early luteal 134

Mid luteal 140

Late luteal 96

Hello Stefan and thank you very much for the response.

Would you be so kind and tell me the references?

Thank you again!

Hi Sergio

Below is one reference and then we also contacted our local pathologists. They provided us with data from their blood analysis records - perhaps you can do that as well.

Yeung EH, Zhang C, Mumford SL, Ye A, Trevisan M, Chen L, et al. Longitudinal study of insulin resistance and sex hormones over the menstrual cycle: the BioCycle Study. J Clin Endocrinol Metab 2010 Dec;95(12):5435-5442.

Thank you very much!

Dear Sergio,

E2 and P4 values were measured in porcine oviduct fluid

Please see this paper from my research group from Murcia Univeresity. Spain

Ballester, L., Romero-Aguirregomezcorta, J., Soriano-Ubeda, C., Matas, C., Romar, R. and Coy, P. (2014) Timing of oviductal fluid collection, steroid concentrations, and sperm preservation method affect porcine in vitro fertilization efficiency. Fertil. Steril., 102, 1762-1768 e1761.

My best regards

Joaquín

Try this.  The Physiology of Reproduction. Volume 1. Editors in Chief - Ernst Knobil and Jimmy D Neill. Chapter 9 The primate oviduct and endometrium. Pages 303-329

The values you want are all in this chapter. Just attaching a page

@ Joaquin Gadea, thank you very much for your interest. The values shown in the article are a great reference for my experiments. I would like to further inquire about the levels found across all sections of the oviduct (istmus, ampulla, fimbria). Do you know whether E2 concentrations significantly vary towards the distal end (istmus) upon ovulation?

@ Rashmikant Patel. thank you!.  The book page shows morphological features across the oviduct (Fig. 9) and table 1, indicates concetrations of E2, but it appears to be for the blood plasma concetrations. Maybe I am missing somethng, since I don't have the book right now.

You are right. The values are serum values. As I understand oestradiol is an endocrine hormone produced in the ovaries with the effects on target organs. One of the target organ is oviducts. I suspect you won't find oestradiol in the tubal secretions. The  function of the oviduct is mainly to culture and transport the fertilized oocyte. Not forgetting the sperm transport.(it is not an autocrine or paracrine effect) 

The culture media used in IVF does not contain any hormones and these culture media were evolved from collection of tubal secretions by  cannulation  of the rabbit and mice oviducts and total hystercectomy specimens of females.

I hope this helps you.