All my Selaginella got contamination after sterilization phase. I already
try to change method for sterile it such :-
1. soak them in fungicide + tween 20
2. rinse with dh2O (3times)
3. dip in 70% ethanol (30second)
4. soak in sodium hypochlorite/clorox (20%-60% concentration) / 0.1% mercury chloride(HgCl2) for 5-10 min.
5. rinse with dH2O (3-5 times)
6. dry it on filter paper
7. put in media
Any suggestion, what should i do to improve it from contamination?
Thank you.