What are the different techniques for splitting the overlapping bacterias?

More Prachi Arora's questions See All

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to validate image labeling quality within dataset?

Hello, it is about creating a dataset for semantic segmentation with three classes. The problem is that one class dominates with >90% and one class is

18 February 2021 4,254 5 View

Using 1D CNN for pixel classification in an image?

I have a 3D Image of size 100x100x100. I have features (feature dimension 25) extracted for each pixel by considering a small pacth around it. So the input to 1D CNN is 100x100x100x25 reshaped to...

17 February 2021 941 4 View

How should I analyze the multiple scans in Malvern Zetasizer software?

Hello, I am currently using the Malvern Zetasizer software for the purpose of obtaining the size distribution of nanoparticles produced in the lab. When I perform the runs in the software to get...

15 February 2021 4,251 1 View

Which optimization algorithm is most efficient for fitting a regression model using training dataset?

The goal of regression model is either to minimize the prediction error or to maximize a classification accuracy depending on the type of the model. Is there any optimization algorithm that is...

10 February 2021 4,128 3 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

Why does the accuracy of NN decreases when two datasets are combined?

I am working on gait analysis using a feed-forward NN. When I use data from the accelerometer (3 columns for each axis if stored in a data frame), I get an accuracy of 95%, and when I used data...

07 February 2021 9,654 6 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Can I know how to apply contourlet transform for signals in MATLAB?Must I first convert 1D signal to 2D image in order to do this method?

01 February 2021 3,735 3 View

Which techniques are used to improve the accuracy of deep learning models by compressing neural networks?

Hi all, I am looking for enhancing the accuracy of a deep learning model using some techniques to compress neural networks. Could someone suggest to me some of the tools/techniques,...

29 January 2021 1,781 4 View

Changming Sun

You can have a look at the following papers:

Article Finding splitting lines for touching cell nuclei with a shor...

Article Clustered nuclei splitting via curvature information and gra...

Article Splitting touching cells based on concave points and ellipse fitting

Abbas Cheddad

Check the following:

RFOVE - Cell Segmentation

C. Panagiotakis and A.A. Argyros, "Region-based Fitting of Overlapping Ellipses and its Application to Cells Segmentation", Image and Vision Computing, Elsevier, vol. 93, pp. 103810, 2020.

https://se.mathworks.com/matlabcentral/fileexchange/74200-rfove-cell-segmentation

And

Steve's blog on Cell segmentation

https://blogs.mathworks.com/steve/2006/06/02/cell-segmentation/