I have isolated a fungi in Rye A agar from infected Capsicum leaf.
The isolated fungi does not producing spore or any fruiting bodies only mycelium is present by microscopic study, when stain with lactophenol cotton blue.
I request researcher to inform me about different techniques in fungal identification like morphological, biochemical etc.
probably you want to sequence pieces of rRNA to get an idea to which major group this fungus belongs (even to species with ITS sequence if you are luck the species has been sequenced), then you may have a clue about how to induce teleomorph/anamorph for further identification to species.
I will go for molecular approach, but initially I want some traditional method for identification of isolated fungi.
Please tell me:
if I use UV what will be the time and wavelength for exposure??
if any conventional biochemical test is there for identification??
if any one having pictorial database, so that I can match the structure please send me!!
When no distinctive structures are present either micro or macroscopically that allow classical identification I would try molecular biology. You can extract the fungus total DNA and then amplified an specific amplicon (ITS) with universal fungal primers such as ITS4 and ITS5. You should find a good database to compare your results against in GeneBank. Using frequently-used primers can be advantageous due to the size of the databank already built by other researchers and might give you a quick answer. Good luck!
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