In general, longer run times with lower voltages tend to produce better gels.
Combination of morphometric and colorimetric has been applied for feature extraction in detection algorithm. In noisy background, this CV technique facing wrong identification results as well as...
07 August 2018 7,773 7 View
Filtering dataset quality is very important prior to segmentation analysis. A single channel of an image is applied before convolve it with the Laplacian kernel.
31 December 2017 9,956 0 View
This is specific to the face recognition in unconstrained environment, generalization capability of the face recognition systems with large training datasets and generation of additional synthetic...
11 December 2017 1,090 2 View
The same strel is apply for dilation and erosion operation in morphological operator.
11 December 2017 661 0 View
This subject is discussed under indexing in MATLAB. Basic script, Img (Img=0) = 1 (All zero intensities changed to one), or can use Img>200, depending on the aforementioned tech. used (one can...
03 April 2017 4,793 4 View
It was reported as a basic procedure in the software eCognition for objectoriented image analysis (Delphi2 Creative Technologies, Munich).
03 April 2017 3,385 0 View
It is more realistic to model pixels in an images with some degree or type of correlation, because due to the point spread function and the intensity of a pixel may contain info from its...
03 April 2017 2,298 0 View
If a part of the energy that an electron carries is transferred to the specimen, several processes might occur leading to the generation of the above signals.
31 December 2016 5,725 0 View
To understand basic of various spectroscopy techniques, type of detector of electron with characteristic kinetic energy is important to be explained.
11 December 2016 447 0 View
Is there any formula or specific program?
11 December 2016 642 6 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.
07 August 2024 9,508 1 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...
01 August 2024 2,614 1 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
How to change the displayed full article text to its corrected version? In the file on the page of the journal where I published the article, there was an error in the text, the table is...
30 July 2024 3,229 2 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...
30 July 2024 3,126 0 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View