Can someone please tell me do moderation analysis have IV and DV and what are they?

H1 - It is hypothesised that individuals with poorer mental health will be associated with increased offending behaviours. H2 It is further hypothesised that Dark triad traits will moderate the...

04 September 2020 403 4 View

Are there bioinformatics tools that can predict change in secondary structure?

I am curious if there exist any bioinformatics tools that can predict changes in secondary structure for proteins and/or nucleic acids. For instance, say a C-terminal loop on a protein reorganizes...

19 May 2020 1,963 8 View

My primer has some homology with a sequence within my 3.2kb PCR product, and keep producing 700bp bands and very faint ones at 3.2kb. How to fix this?

I am trying to PCR amplify a 3.2kb region from a plasmid so that i can use gibson assembly to put it into a different one. The desired insert has 3 genes and 2 RBS sites, one of which shares some...

24 February 2019 2,866 3 View

How do I test for Linearity when I have 1 dv and 3 DV's?

Hi there, I am trying to test for linearity with my 1 iv and 3 dv. I go to Analyze>regression>linear , but in the dependent box you can only put 1 dv. I am very new to spss and am not sure...

14 May 2018 9,733 3 View

I know the amine number of a polymer, and the viscosity range. How do I determine the equivalent weight of Amine?

29 January 2017 7,938 2 View

Troubleshooting Overnight Rest of PBMCs in Serum free Media?

I am working on a protocol to stimulate PBMCs with IFNy to measure transcription activation of genes along the kynurenine pathway using rt-PCR. I want to use serum free media to minimize...

13 June 2016 1,592 3 View

Is there a problem with buffer as I get no signal with 2D-PAGE or Western blot ?

I am trying to measure phosphorylation of lamin A subunits by 2D-PAGE. I resuspend Vero cells in Rehydration buffer (which contains 8M urea) and sonicate them. I run this cell lysate sample on an...

26 October 2015 3,471 2 View

Hello, What are the steps that a teacher (or school) needs to take to prepare for and then implement PhoneLabs? are pilot opportunities possible?

Before a strategy within a school needs to be developed, there needs to be a strategy and support mechanisms put in place for a disruptive champion (probably a single teacher) to get the seed...

01 January 1970 9,566 3 View

The ISLC in Edinburgh titled "Rethinking Leadership Research". Are there available papers reflecting this title?

I'm interested in papers from the ISLC in Edinburgh titled "Rethinking Leadership Research". Are there any proceedings or available papers reflecting this title from the conference?

01 January 1970 4,548 3 View

Endocrine Disruption - how far have we advanced?

Dear Colleagues, we would be most grateful if you could complete this very short survey on Endocrine Disrupting Chemicals (EDCs). This survey is intended to inform the wider scientific community...

01 January 1970 8,051 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View