What are the best ways to detect dsDNA in the cytoplasm?

16 October 2017 1 641 Report

re there specific stains and fluorescent methods to detect dsDNA in the cytoplasm of cells? Are these quantitative? What are the best controls and methods to validate dsDNA in cytoplasm?

Aleem Syed

There is a dsDNA specific stain (called PicoGreen from Thermo) that we could use to stain cytosolic DNA. see highlighted text in the attached

https://www.thermofisher.com/order/catalog/product/P7589

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